Abstract ID: 3535

Primary Topic: Whole systems and acupuncture (including TCM and Ayurveda)
Secondary Topic: Mind-body (including meditation and yoga)

Mechanisms underlying the Antidepressant Response of Acupuncture via JNK Singaling Pathway
Qiuyun Yu, master; Tuya Bao, professor; Xinjing Yang, doctor; Huili Jiang, doctor; Bingcong Zhao, doctor, Beijing University of Chinese Medicine, beijing, China

Late Breaker: No

Purpose

 To observe the effect of acupuncture on JNK signaling pathway in rats with chronic unpredicted mind stress (CUMS), so as to reveal the underlying mechanism of acupuncture for depression. 

Methods/Session Format

 80 Sprague-Dawley rats were randomly divided into 8 groups as control, model, model+SP600125 (SP), model+DMSO, Fluoxetine (FLX), FLX+SP, acupuncture and acupuncture+SP (n=10 in each group). The depression-like behavior model was established by CUMS for 21 days. Intracerebroventricular injections of the JNK signaling pathway inhibitor SP600125(10μM,5μL) were administered in model+SP group, FLX+SP group and  acupuncture+SP group. Acupuncture at Neiguan(PC 6) and Sanyinjiao (SP 6) was performed thirty minutes before CUMS procedure daily (20 minutes per session).Rats in FLX group were administered with fluoxetine (0.18 ?/ml) by gavage daily (1ml/100g). Open-field test and body weight were investigated respectively at  day 0 pre-intervention and at day 21 post-intervention. Expression of JNK, c-jun and p-c-jun in the Prefrontal Cortex were detecteded by using western blot. 

Results

 CUMS could induce depressive-like behaviors. The expression of JNK, c-jun and p-c-jun were up-regulated by CUMS in model group, model+SP group and model+DMSO group compared with the control group (P<0.01). Compared with model group, the expression level of each protein in model+DMSO group showed no significant difference (p>0.05). The expression level of JNK in model+SP group, FLX group and FLX+SP group showed no significant difference compared with model group (p>0.05), while in acupuncture group and acupuncture+SP group, JNK expression level showed significant difference (p<0.01). The expression level of c-jun was reduced significantly in model+SP group, FLX group, FLX+SP group, acupuncture group and acupuncture+SP group (p<0.05).The expression level of p-c-jun was reduced significantly in FLX group, FLX+SP group, acupuncture group and acupuncture+SP group (p<0.01). Compared with FLX group, the expression of the three proteins in FLX+SP group showed no significant difference (p>0.05). Compared with acupuncture group, the expression of the three proteins in acupuncture+SP group also showed no significant difference (p>0.05).

Conclusions

Acupuncture could ameliorate depressive-like behaviors by regulating related proteins on the JNK signaling pathway in the Prefrontal Cortex.