Whole systems and acupuncture (including TCM and Ayurveda)
Secondary Topic: Mind-body (including meditation and yoga)
Mechanisms underlying the Antidepressant Response of Acupuncture via JNK Singaling Pathway
Qiuyun Yu, master; Tuya Bao, professor; Xinjing Yang, doctor; Huili Jiang, doctor; Bingcong Zhao, doctor, Beijing University of Chinese Medicine, beijing, China
Late Breaker: No
To observe the effect of acupuncture on JNK signaling pathway in rats with chronic unpredicted mind stress (CUMS), so as to reveal the underlying mechanism of acupuncture for depression.
80 Sprague-Dawley rats were randomly divided into 8 groups as control, model, model+SP600125 (SP), model+DMSO, Fluoxetine (FLX), FLX+SP, acupuncture and acupuncture+SP (n=10 in each group). The depression-like behavior model was established by CUMS for 21 days. Intracerebroventricular injections of the JNK signaling pathway inhibitor SP600125(10μM,5μL) were administered in model+SP group, FLX+SP group and acupuncture+SP group. Acupuncture at Neiguan(PC 6) and Sanyinjiao (SP 6) was performed thirty minutes before CUMS procedure daily (20 minutes per session).Rats in FLX group were administered with fluoxetine (0.18 ?/ml) by gavage daily (1ml/100g). Open-field test and body weight were investigated respectively at day 0 pre-intervention and at day 21 post-intervention. Expression of JNK, c-jun and p-c-jun in the Prefrontal Cortex were detecteded by using western blot.
CUMS could induce depressive-like behaviors. The expression of JNK, c-jun and p-c-jun were up-regulated by CUMS in model group, model+SP group and model+DMSO group compared with the control group (P<0.01). Compared with model group, the expression level of each protein in model+DMSO group showed no significant difference (p>0.05). The expression level of JNK in model+SP group, FLX group and FLX+SP group showed no significant difference compared with model group (p>0.05), while in acupuncture group and acupuncture+SP group, JNK expression level showed significant difference (p<0.01). The expression level of c-jun was reduced significantly in model+SP group, FLX group, FLX+SP group, acupuncture group and acupuncture+SP group (p<0.05).The expression level of p-c-jun was reduced significantly in FLX group, FLX+SP group, acupuncture group and acupuncture+SP group (p<0.01). Compared with FLX group, the expression of the three proteins in FLX+SP group showed no significant difference (p>0.05). Compared with acupuncture group, the expression of the three proteins in acupuncture+SP group also showed no significant difference (p>0.05).
Acupuncture could ameliorate depressive-like behaviors by regulating related proteins on the JNK signaling pathway in the Prefrontal Cortex.